Cell Dilution Calculator

Your details

Choose which variable to calculate. Fill in the other three.
Cell density of your stock suspension, in cells per mL.
cells/mL
Desired cell density in the final diluted suspension.
cells/mL
Total volume of the final diluted suspension.
mL
Calculated valueModerate dilution (2-10 fold)
0.5

The missing variable solved from C1 x V1 = C2 x V2

UnitmL
Dilution factor0.25
Dilution ratio1:4
Diluent to add1.5mL
Total cells in final suspension1.00 x 10^6 cells
Dilution factor (C2/C1)0.25

1:4 dilution calculated successfully.

  • Pipette 0.5000 mL from your stock, then add 1.5000 mL of media or buffer to reach your target volume.
  • Dilution factor is 0.2500 (1:4), meaning the final suspension is 4.0x less concentrated than your stock.
  • Total cells in the prepared suspension: 1.00 x 10^6 cells.

Next stepMix gently by inversion or pipetting (avoid vortexing mammalian cells), then verify the actual concentration on a hemocytometer or automated cell counter before use.

 = Powered by OnlyCalculators