DNA Copy Number Calculator

Your details

Forward: how many copies per uL does your stock contain? Reverse: how many ng do you need per uL to hit a target copy number?
Average molecular weight per base pair (dsDNA = 660 Da, ssDNA = 330 Da, ssRNA = 340 Da).
Measured concentration of your DNA or RNA stock, typically from a NanoDrop or fluorometer.
ng/uL
Length of your DNA or RNA molecule in base pairs (for dsDNA) or bases (for ssDNA/ssRNA).
bp
Project how many copies you will have after a given number of PCR cycles, assuming 100% efficiency.
Copy numberHigh copy number
9,124,242,424.242copies/uL

Number of DNA molecules per microliter

Copy number (readable)9.12 billion
Molar mass of fragment660,000Da
Moles per uL1.515e+4 amol/uL
Initial copies/uL9,124,242,424.242
Copies after PCR-

9.12 billion copies/uL in a 1000 bp double-stranded DNA fragment.

  • At 10 ng/uL, a 1000 bp double-stranded DNA fragment contains approximately 9.12 billion copies per microliter.
  • The molar mass of this fragment is 660,000 Da, calculated as 1000 bp x 660 Da/bp.
  • Concentration accuracy depends on your measurement method: fluorometric methods (Qubit) are more DNA-specific than absorbance methods (NanoDrop), which can overestimate if contaminants are present.

Next stepUse the reverse mode to calculate the ng/uL needed for a specific copy number target, such as a qPCR standard curve starting point.

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