qPCR Efficiency Calculator

Your details

Slope method: enter the gradient from your Ct-vs-log(concentration) plot. Delta Ct method: enter two Ct values from a known dilution.
The gradient of the Ct vs. log(copy number) standard curve. Should be negative and close to -3.32 for 100 % efficiency.
Goodness-of-fit of the standard curve. MIQE guidelines require R² ≥ 0.98 for the assay to be considered reliable.
EfficiencyAcceptable (90-110 %)
100.08%

Amplification efficiency of the qPCR assay

Amplification factor2.0008
Derived slope-3.32
R² entered0.998
Quality ratingAcceptable - meets MIQE guidelines
100.08 %
Too low<80Below range80-90Acceptable90-110Above range110-120Too high120+

qPCR efficiency: 100.1 % - within acceptable range

  • Efficiency of 100.1 % is within the MIQE-recommended 90-110 % range. Your assay is performing reliably.
  • Amplification factor: 2.001. At 100 % efficiency the factor is exactly 2.000, meaning the template doubles each cycle.
  • Derived slope of -3.320 falls in the target range of -3.1 to -3.6.
  • R² of 0.998 meets the MIQE threshold of ≥ 0.98, confirming a well-fitted standard curve.

Next stepThis assay passes MIQE efficiency criteria. Proceed with relative quantification (delta-delta Ct) or absolute quantification against this standard curve.

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