Serial Dilution Calculator

Your details

Dilution factor mode: you pick the same fold-reduction per step. Concentration range mode: you set the start and final concentration and the calculator derives the factor.
Concentration of the original stock solution before any dilution.
Each tube is diluted by this factor. A factor of 10 means each step is 1/10 of the previous concentration.
How many tubes in the serial dilution series (2 to 20).
Total volume in each dilution tube. The transfer volume is drawn from this.
Dilution factor per stepStandard dilution (1:10)
10

The fold-dilution applied at each transfer step

Transfer volume per step0.1
Diluent volume per step0.9
Final concentration (last step)0.01
Total diluent needed5.4
Cumulative dilution factor (final step)100,000
Transfer volume0.1
Diluent volume0.9

1:10 serial dilution over 6 steps

  • Each step transfers 0.1 mL into 0.9 mL of diluent, giving a 1:10 dilution per tube.
  • After 6 steps, the concentration falls from 1000 to 0.01 µmol/L (µM) - a 100000-fold total dilution.
  • Transfer volumes are within a comfortable pipetting range. Use a calibrated pipette and mix thoroughly after each transfer to minimise carry-over error.

Next stepPrepare all tubes with diluent first, then transfer in sequence from highest to lowest concentration. Change tips between every transfer to avoid carry-over.

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